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Histone H3 (trimethyl K36) Rabbit, Clone: RM155, Abnova™

Rabbit monoclonal antibody raised against synthetic trimethyl peptide of human histone H3 (trimethyl K36).

Manufacturer:  Abnova Corporation MAB12835

Catalog No. 89-947-508

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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]



Histone H3 (trimethyl K36)
Rabbit monoclonal antibody raised against synthetic trimethyl peptide of human histone H3 (trimethyl K36).
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
100 ug
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
This antibody reacts to histone H3 trimethylated at Lysine 36. No cross reactivity with monomethylated Lysine 36 or dimethylated Lysine 36, or other methylations in histone H3.
ChIP assay, ELISA, Western Blot
ChIP (2-10 ug/mL) ELISA (0.2-1 ug/mL) Western Blot (0.5-2 ug/mL) The optimal working dilution should be determined by the end user.
A synthetic trimethyl peptide corresponding to residues surrounding K36 of human histone H3.
Protein A affinity purification
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