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GE Healthcare HisTrap FF

Ensure fast, reliable capture of histidine-tagged proteins in IMAC. GE Healthcare HisTrap FF are ready-to-use columns for preparative purification. It is the product of choice when charging the medium with different metal ions for optimization of purification protocol.

Manufacturer:  GE Healthcare 17525502

Catalog No. 45-002-373

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
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Description

Description

HisTrap FF is a ready-to-use column, pre-packed with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). The high flow rates made possible by the Ni Sepharose 6 Fast Flow matrix make HisTrap FF columns well suited for small scale-up.
  • High binding capacity—approx. 40mg/ml medium
  • Negligible leakage of Ni2+
  • Convenient pre-packed columns offer reliable and time-saving purification
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • Capacity can be increased by connecting columns in a series
Ni Sepharose 6 Fast Flow consists of 90µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.
Specifications

Specifications

1mL
HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Polypropylene (PP)
100 x 5 mL
HisTrap FF
100 x 5mL
≈40 mg histidine-tagged protein /mL medium
< 4 mL/min
6% cross-linked agarose
90μm, d50v is the median particle size of the cumulative volume distribution.
5 bar [0.5 MPa] (70 psi)
4 to 30°C, 20% Ethanol
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