GE Healthcare HisTrap FF
Ensure fast, reliable capture of histidine-tagged proteins in IMAC. GE Healthcare HisTrap FF are ready-to-use columns for preparative purification. It is the product of choice when charging the medium with different metal ions for optimization of purification protocol.
Manufacturer: GE Healthcare 17525502
DescriptionHisTrap FF is a ready-to-use column, pre-packed with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). The high flow rates made possible by the Ni Sepharose 6 Fast Flow matrix make HisTrap FF columns well suited for small scale-up.
- High binding capacityapprox. 40mg/ml medium
- Negligible leakage of Ni2+
- Convenient pre-packed columns offer reliable and time-saving purification
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives
- Capacity can be increased by connecting columns in a series
|HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).|
|100 x 5 mL|
|100 x 5mL|
|≈40 mg histidine-tagged protein /mL medium|
|< 4 mL/min|
|6% cross-linked agarose|
|90μm, d50v is the median particle size of the cumulative volume distribution.|
|5 bar [0.5 MPa] (70 psi)|
|4 to 30°C, 20% Ethanol|
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