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GE Healthcare HisTrap FF Crude, 100 x 5ml

Enjoy simple, one-step purification with a minimum of sample prep and equipment. GE Healthcare HisTrap FF Crude, 100 x 5ml are prepacked columns that purify histidine-tagged proteins directly from homogenized, unclarified cell lysate by immobilized metal ion affinity chromatography (IMAC).

Manufacturer:  GE Healthcare 17528602

Catalog No. 45-002-374

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

HisTrap FF crude is a ready-to-use column filled with precharged Ni Sepharose 6 Fast Flow. The special design of the column in combination with the medium, provides fast, simple, and convenient purifications. After thorough cell disruption, it is possible to load the unclarified lysate on the column without precentrifugation and filtration of the sample.Short purification time generally minimizes deleterious effects, such as degradation and oxidation of sensitive target proteins, and is therefore of great importance. HisTrap FF crude columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as Ä;KTA design chromatography systems.
  • Optimized for purification of histidine-tagged proteins directly from homogenized, unclarified cell lysates
  • High binding capacity, approx. 40mg/ml medium and negligible Ni2+ leakage
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • Reduced sample preparation time, which minimizes risk of protein degradation by proteases
  • No need for centrifugation or filtration of the samples; direct sample application
  • Simple operation with a syringe, pump or chromatography system, such as Ä;KTA design or FPLC System
  • Permits rapid yet reliable separations with a minimum of sample preparation and equipment—simply sonicate and run
Extending the duration of the mechanical treatment of the sample to ensure a more complete lysis is recommended. For optimal results, it is recommended that lysozyme and DNase I are added, followed by a mechanical lysis (for example, by sonication). The homogenized sample can then be loaded directly on the column without a clarification step which may prevent degradation of the target protein and increase the activity.
Specifications

Specifications

5mL
≈40 mg histidine-tagged protein/ml medium, Protein binding capacity is protein-to-protein dependent.
< 20 mL/min
Polypropylene (PP)
100 x 5 mL
HisTrap FF Crude, 100 x 5 mL
100 x 5mL
≈40 mg histidine-tagged protein /mL medium
HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.
Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes
6% cross-linked agarose
90μm, d50v is the median particle size of the cumulative volume distribution.
5 bar [0.5 MPa] (70 psi)
4 to 30°C, 20% Ethanol
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