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GE Healthcare HisTrap HP, 100 Columns

Ensure reliable, high-yield capture of histidine-tagged proteins in IMAC. GE Healthcare HisTrap HP, 100 Columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification by immobilized metal ion affinity chromatography (IMAC).

Manufacturer:  GE Healthcare 17524805

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Catalog No. 45-002-372

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HisTrap HP 1ml and 5ml columns are designed for one-step purification of histidine-tagged proteins. The columns are pre-packed with Ni Sepharose High Performance to ensure reliable capture of target protein in repeated IMAC purifications. The high stability and broad compatibility of Ni Sepharose High Performance maintains biological activity and increases product yield. At the same time, it greatly expands the range of suitable operating conditions.
  • High binding capacity—at least 40mg/ml chromatography medium
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • Negligible Ni2+ leakage
  • Simple manual operation with a syringe, pump or chromatography system, such as Ä;KTA design
  • Can be used for purification of a range of tagged proteins
  • 100-column bulk pack for convenience and economy
HisTrap HP columns can also be used for the purification of tagged proteins containing shorter or longer polyhistidine tags, such as (histidine)4 or (histidine)10. The shorter (histidine)4 will bind more weakly and the longer (histidine)10 will bind more strongly compared with (histidine)6. This difference in binding strength can be used during purification; since (histidine)10 binds more strongly, a higher concentration of imidazole can be added to the lysed cells. This can facilitate the removal of contaminants that can otherwise be co-purified with the tagged target protein.For convenient scaling up of histidine-tagged protein purification, use 20 ml HisPrep FF 16/10 columns. Ni Sepharose 6 Fast Flow, the medium prepacked in HisTrap FF and HisPrep FF 16/10 columns, allows high flow rates, which facilitates scale-up of histidine-tagged protein purification.


At least 40 mg histidine-tagged protein/ml medium, Protein binding capacity is protein-to-protein dependent.
4 mL/min (1 mL), 5 mL/min (5ml)
Highly cross-linked agarose, 6%
34μm, d50v is the median particle size of the cumulative volume distribution.
5 bar [0.5 MPa] (70 psi)
4 to 30°C, 20% Ethanol
At least 40 mg histidine-tagged protein/ml medium, Protein binding capacity is protein-to-protein dependent.
HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Polypropylene (PP)
100 x 5 mL
HisTrap HP, 100 x 5 mL
100 x 5mL
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