Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

GE Healthcare HisTrap HP, 100 Columns

Ensure reliable, high-yield capture of histidine-tagged proteins in IMAC. GE Healthcare HisTrap HP, 100 Columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification by immobilized metal ion affinity chromatography (IMAC).

Specifications

Flow Rate 4 mL/min (1 mL), 5 mL/min (5ml)
Matrix Highly cross-linked agarose, 6%
Storage Requirements 4 to 30°C, 20% Ethanol
Capacity (Metric) At least 40 mg histidine-tagged protein/ml medium, Protein binding capacity is protein-to-protein dependent.
Particle Size 34μm, d50v is the median particle size of the cumulative volume distribution.
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Bed Volume Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Bed Volume Price Quantity & Availability  
45-002-371 View Documents GE Healthcare
17524705
100 x 1mL 1mL This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
View substitute products »
45-002-372 View Documents GE Healthcare
17524805
100 x 5mL 5mL This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
View substitute products »
Description

Description

HisTrap HP 1ml and 5ml columns are designed for one-step purification of histidine-tagged proteins. The columns are pre-packed with Ni Sepharose High Performance to ensure reliable capture of target protein in repeated IMAC purifications. The high stability and broad compatibility of Ni Sepharose High Performance maintains biological activity and increases product yield. At the same time, it greatly expands the range of suitable operating conditions.
  • High binding capacity—at least 40mg/ml chromatography medium
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • Negligible Ni2+ leakage
  • Simple manual operation with a syringe, pump or chromatography system, such as Ä;KTA design
  • Can be used for purification of a range of tagged proteins
  • 100-column bulk pack for convenience and economy
HisTrap HP columns can also be used for the purification of tagged proteins containing shorter or longer polyhistidine tags, such as (histidine)4 or (histidine)10. The shorter (histidine)4 will bind more weakly and the longer (histidine)10 will bind more strongly compared with (histidine)6. This difference in binding strength can be used during purification; since (histidine)10 binds more strongly, a higher concentration of imidazole can be added to the lysed cells. This can facilitate the removal of contaminants that can otherwise be co-purified with the tagged target protein.For convenient scaling up of histidine-tagged protein purification, use 20 ml HisPrep FF 16/10 columns. Ni Sepharose 6 Fast Flow, the medium prepacked in HisTrap FF and HisPrep FF 16/10 columns, allows high flow rates, which facilitates scale-up of histidine-tagged protein purification.
Specifications

Specifications

4 mL/min (1 mL), 5 mL/min (5ml)
4 to 30°C, 20% Ethanol
34μm, d50v is the median particle size of the cumulative volume distribution.
Highly cross-linked agarose, 6%
At least 40 mg histidine-tagged protein/ml medium, Protein binding capacity is protein-to-protein dependent.
Polypropylene (PP)
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit