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Cytiva HiTrap™ MabSelect SuRe™ PCC Columns
Prepacked with MabSelect SuRe™ PCC Protein A Resin for screening and small-scale mAb purification in continuous bioprocessing using periodic counter-current chromatography (PCC)
Supplier: Cytiva 17549112
Description
- Suited for small scale antibody purifications, as well as optimization of methods and parameters, such as sample load and protein binding conditions
- Alkali-stabilized, protein A-derived ligand gives the resin greater stability than conventional protein A-based resins under the alkaline conditions used in cleaning-in-place (CIP) protocols
- These protein A columns provide fast, reproducible, and easy separations in a convenient format
- Highly concentrated elution pools for operating flexibility and small unit operations
- Allows cost-effective cleaning with 0.1 to 0.5 M NaOH over hundreds of purification cycles
- Efficiently inactivates bacteria, mold, and yeast, with improved product quality as a result
- Columns are optimally utilized when they are connected to liquid chromatography systems such as the ÄKTA systems
- High binding capacity: ∼ 60 mg Human IgG/mL Resin at 2.4 min residence time
Specifications
Flow Velocity: ≤ 600 cm/h | |
5 mL | |
1/Pk. | |
<20 mL/min | |
Store at 2°C to 8°C, 20% Ethanol | |
Affinity Column | |
MabSelect SuRe PCC Protein A Resin |
Chromatography Column | |
16 mm | |
25 mm | |
5 bar (0.5 MPa) | |
∼60 mg Human IgG/mL Resin | |
Antibody Affinity Chromatography |
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