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HLA-A2 Mouse anti-Human, PerCP-eFluor™ 710, Clone: BB7.2, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 46987642

Catalog No. 50-112-3982


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Description

Description

Description: This BB7.2 monoclonal antibody recognizes the human major histocompatibility complex (MHC) class I molecule HLA-A2, which is a member of the HLA-A family. MHC class I exists as a single alpha chain composed of three domains. This molecule associates with beta 2-microglobulin and is expressed on all nucleated cells. The BB7.2 monoclonal antibody has been reported to exhibit blocking activity, as well as recognize HLA-A28. Applications Reported: This BB7.2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This BB7.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat.

00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.
Specifications

Specifications

HLA-A2
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P05534
DAQB-90C11.16, FLJ26655, HLAA
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
BB7.2
PerCP-eFluor™ 710
HLA-A
Liquid
HLA-A
IgG2b, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
3105
Documents
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