HLA-G, Mouse anti-Human, Clone: 4H84, Millipore Sigma™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
MABF216925	MilliporeSigma MABF216925UG	25 μg
MABF2169100	MilliporeSigma MABF2169100UG	100 μg

Description

Anti-HLA-G, clone 4H84, Cat. No. MABF2169, is a mouse monoclonal antibody that detects HLA class I histocompatibility antigen, alpha chain G and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.

HLA class I histocompatibility antigen, alpha chain G (UniProt: P17693; also known as HLA G antigen, MHC class I antigen G) is encoded by the HLA-G (also known as HLA-6.0, HLAG) gene (Gene ID: 3135) in human. HLA-G is a single-pass type I membrane glycoprotein that serves as marker of extravillous cytotrophoblasts in placenta and plays a central role in molecule maternal tolerance of the semiallogeneic fetus. It is involved in the presentation of foreign antigens to the immune system. HLA-G is a heterodimer of an alpha chain and a beta chain. Severn different isoforms of HLA-G have been described that are produced by alternative splicing and their distribution is temporally affected during pregnancy in placenta and fetal tissues. HLA-G expression has been reported in renal cell carcinoma where it is exclusively restricted to tumor cells. Its expression is thought to be involved in the impairment of the anti-tumor immunity or resistance to immunotherapeutic agents. HLA-G is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form, which contains an extracellular domain (aa 25-308), a transmembrane domain (aa 309-332), and a short cytoplasmic tail (aa 333-338).

Specifications

• Antigen: HLA-G
• Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected HLA-G in human spleen tissue sections.Immunohistochemistry (Paraffin) Analysis: A representative lot detected HLA-G in Immunohistochemistry applications (Tabata, T., et. al. (2012). Am J Pathol. 181(5):1540-59; Menier, C., et. al. (2003). Hum Immunol. 64(3):315-26; Ibrahim, E.C., et. al. (2003). Am J Pathol. 162(2):501-8; Tabata, T., et. al. (2016). Cell Host Microbe. 20(2):155-66; McMaster, M., et. al. (1998). J Immunol. 160(12):5922-8).Western Blotting Analysis: A representative lot detected HLA-G in Western Blotting applications (Paul, P., et. al. (1999). Cancer Res. 59(8):1954-60; McMaster, M., et. al. (1998). J Immunol. 160(12):5922-8).ELISA Analysis: A representative lot detected HLA-G in ELISA applications (McMaster, M., et. al. (1998). J Immunol. 160(12):5922-8).Flow Cytometry Analysis: A representative lot detected HLA-G in Flow Cytometry applications (Menier, C., et. al. (2003). Hum Immunol. 64(3):315-26).
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: HLA class I histocompatibility antigen alpha chain G;HLA G antigen;MHC class I antigen G
• Gene Symbols: HLA-G;HLA-6.0;HLAG
• Isotype: IgG1 κ
• Purification Method: Protein G purified
• Test Specificity: Clone 4H84 specifically detects human HLA-G. It recognizes an epitope with in 23 amino acids from the alpha1 region of all HLA-G isoforms.

• Clone: 4H84
• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Inflammation & Immunology
• Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene ID (Entrez): NP_002118.1
• Immunogen: KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal region of Human MHC class I antigen G. The immunogen sequence is conserved in all isoforms.
• Primary or Secondary: Primary
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.