Hobit (ZNF683) Mouse anti-Human, Alexa Fluor 647, Clone: Sanquin-Hobit/1, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 566250

Catalog No. BDB566250

Description

The Sanquin-Hobit/1 monoclonal antibody specifically binds to Homolog of Blimp-1 in T cells (Hobit), which is also known as Zinc finger protein 683 (ZNF683). Hobit is a conserved Zn-finger transcription factor that is homologous to Blimp-1. It is expressed in cytotoxic effector-type CD8+ T cells and in some effector memory CD8+ T cells, but is not significantly expressed in naοve or most central memory CD8+ T cells. Hobit is also expressed in natural killer (NK) cells, and NKT cells, but is not expressed in CD4+ T cells, B cells, monocytes, dendritic cells, or neutrophils. Hobit expression is strongly correlated with the expression T-bet and Interferon-γ(IFN-γ) mRNA. Hobit appears to play a role in the differentiation and effector functions of NK cells and effector CD8+ T cells such as in the production of IFN-γ.

Alexa Fluor™ 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor™ 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor™ 647 cannot be used simultaneously.

Specifications

• Antigen: Hobit (ZNF683)
• Conjugate: Alexa Fluor 647
• Format: Affinity Purified
• Host Species: Murine
• Isotype: Mouse BALB/c IgM, κ
• Quantity: 50 Tests
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal

• Clone: Sanquin-Hobit/1
• Formulation: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
• Gene Alias: HOBIT, Homolog of Blimp-1 in T cells, Zinc finger protein 683, ZNF683
• Immunogen: Human Hobit Recombinant Protein
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor™ 647 under optimum conditions, and unreacted Alexa Fluor™ 647 was removed.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human