BAH-1 Mouse anti-Human, Brilliant Violet 421, Clone: BAH-1 (also known as BAH1), BD Optibuild™
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 743955
The BAH-1 monoclonal antibody was raised against human megakaryocytic cells. It was originally proposed that clone BAH-1 was specific for human CD110, also known as c-Mpl or TPO-R. Data has emerged to suggest that BAH-1 antibody may not recognize CD110. However, there is strong evidence that it recognizes an antigen that is highly expressed on human Megakaryocyte-Erythroid Progenitor (MEP) cells. In combination with CD71, BAH-1 expression has been used to distinguish subsets of Hematopoietic Stem Cells and Myeloid Progenitors in human fetal liver, cord blood, and adult bone marrow. BAH-1 antibody can induce megakaryocytopoiesis of both mouse and human bone marrow cells in vitro.The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.
|Human Megakaryocytic Cells|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.|
|BAH-1 (also known as BAH1)|
|Brilliant Violet 421|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|Mouse BALB/c IgG1, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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