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CD79B Mouse anti-Human, BB515, Clone: 3A2-2E7, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 565225

Catalog No. BDB565225

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Immunoglobulin (Ig) antigen receptors are composed of a non-covalently-associated complex of Ig and two other proteins, Ig α and Ig β , clustered as CD79a and CD79b, respectively. CD79b is a membrane glycoprotein of 229 residues, with a predicted relative molecular mass of 36-40 kDa. Its expression is restricted to B lineage cells, has been reported to associate with surface IgM receptor, and may be involved in signal transduction.

Antibody 3A2-2E7 (also known as SN8) was generated by immunization of cell membrane preparations from B-prolymphocytic leukemia cells. It has similar reactivity characteristics as clone CB3-1 (another CD79b available from BD Biosciences Pharmingen, Cat. No. 555678). Antibodies CD3-1 and 3A2-2E7 reacts with an epitope that is enhanced on certain B-cell leukemias such as prolymphocytic leukemia and lymphoma, but not on chronic lymphocytic leukemia.

The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g. 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.



Affinity Purified
Cell membranes from human B-prol ymphocytic leukemia (B-PLL) cells
Affinity Chromatography
Flow Cytometry
Aqueous buffered solution containing ≤0.09% sodium azide.
IgG1, κ
100 Tests
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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