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ACROBiosystems Recombinant Protein;Human TIGIT Protein, Fc Tag;HEK293;1MG;TIT,TIGIT,VSIG9,VSTM3,VSIG-9,VSTM-3
Supplier: ACROBiosystems TITH52541MG
Recombinant Protein;Human TIGIT Protein, Fc Tag;1MG;Human TIGIT, Fc Tag (TIT-H5254) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1 -1).;This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 39.7 kDa. The protein migrates as 42-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human CD155, mouse IgG2a Fc tag, low endotoxin (Cat. No. CD5-H5254) at 5 μg/mL (100 μL/well) can bind Human TIGIT, Fc Tag (Cat. No. TIT-H5254) with a linear range of 0.6-5 ng/mL (QC tested).;Immobilized Human TIGIT, Fc Tag (Cat. No. TIT-H5254) at 10 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Fc Tag (Cat. No. CD2-H82F8) with a linear range of 0.313-10 μg/mL (Routinely tested).;FACS assay shows that recombinant Human TIGIT Protein, Fc Tag (Cat. No. TIT-H5254) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.1 μg/ml (Routinely tested).
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