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Abnova™ Human ABL1 (NP_005148.2, 2 a.a. - 1130 a.a.) E255K mutant Full length Recombinant Protein with His tag expressed in Baculovirus infected Sf21 cells

Used for Func, SDS-PAGE

Manufacturer:  Abnova™ P5740

Catalog No. 89-932-210


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Description

Description

The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq]

Specifications

Specifications

Baculovirus infected insect cell (Sf21) expression system
Functional Study, Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis
In 50mM Tris-HCl, 150mM NaCl, pH 7.5 (0.05% Brij35, 1mM DTT, 10% glycerol)
126kDa
Baculovirus infected insect cell (Sf21) expression system
78% by SDS-PAGE/CBB staining
5 ug
RUO
ABL1
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : ABLtide. ATP: 100 µM.
Yes
NP_005148.2
Liquid
25
ABL1 (E255K) (Human) Recombinant Protein
Ni-NTA affinity chromatography
SDS-PAGE Stained with Coomassie Blue
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
ABL/JTK7/bcr/abl/c-ABL/p150/v-abl
ABL1
Insect
His
SDS
Documents
Product Certifications
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