Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Abnova™ Human AURKB (Q96GD4, 1 a.a. - 344 a.a.) Full length Recombinant Protein with GST-tag co-expressed with His-tagged INCENP (AAU04398.1, 803 a.a. - 918 a.a.) Full length Recombinant Protein in Baculovirus infected Sf21 cells

Used for Func, SDS-PAGE

Manufacturer:  Abnova™ P5781

Catalog No. 89-936-175


Add to cart

Description

Description

In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM]

Specifications

Specifications

Baculovirus infected insect cell (Sf21) expression system
Functional Study, Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis
In 50mM Tris-HCl, 150mM NaCl, pH 7.5 (0.05% Brij35, 1mM DTT, 10% glycerol)
66kDa
Baculovirus infected insect cell (Sf21) expression system
86% by SDS-PAGE/CBB staining
5 ug
RUO
AURKB
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : Kemptide. ATP: 100 µM.
Yes
Q96GD4
Liquid
9212/3619
AURKB/INCENP (Human) Recombinant Protein
Glutathione sepharose chromatography
SDS-PAGE Stained with Coomassie Blue
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
AIK2/AIM-1/AIM1/ARK2/AurB/IPL1/STK12/STK5
AURKB
Insect
GST
SDS
Documents
Product Certifications
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit