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Invitrogen™ MIP-3 beta Human Uncoated ELISA Kit with Plates Non-distribution product as customer accommodation.

Uncoated ELISA Kit with Plates

Manufacturer:  Invitrogen™ 885210722

Catalog No. 50-112-5169


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Includes: Capture Antibody Concentrate
Detection Antibody Concentrate
Human CCL19 (MCP-3) Standard (lyophilized): 8000pg/mL upon reconstitution
Coating Buffer Concentrate
Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)
Sample Diluent Z
pre-titrated Streptavidin-HRP
Tetramethylbenzidine (TMB) Substrate Solution

Description

Description

The Human Macrophage Inflammatory Protein 3 beta (CCL19) (MIP-3β) Uncoated ELISA Kit contains pre-matched antibody pairs ,plates, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human MIP-3β). Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

CCL19, also known as MIP-3 beta (Macrophage Inflammatory Protein 3 beta), is a member of the CC- subfamily of chemokines. It is most closely related to CCL21, with which it shares 32% amino acid sequence homology. CCL19 and CCL21 are expressed mainly by stromal cells in the T cell-rich zones of lymph nodes. They are critical mediators of the homeostatic trafficking of naive T cells and activated dendritic cells into the secondary lymphoid organs. CCL19 and CCL21 also play a role in T cell priming and activation, as well as the recruitment of lymphocytes to inflamed tissue. Both proteins signal via the G protein-coupled receptor, CCR7, which is expressed on T cells and mature dendritic cells. Although CCL19 and CCL21 both exhibit the same affinity for CCR7, only the binding of CCL19 results in the desensitization and internalization of the receptor.
Specifications

Specifications

Q99731
Biotin
6363
Colorimetric Microplate Reader
ELISA Kit
RUO
Plasma,Serum,Supernatant
Plasma,50 μL|Serum,50 μL|Supernatant,50 μL
2-8°C
24 hr 30 min
CCL19
HRP
63-4,000 pg/mL
ELISA
96-well plates
10X Coating Buffer: Buffer for plating the Capture Antibody
2 x 96 tests
63 pg/mL
50μL
Human
1 hr 45 min
Human
C-C Motif Chemokine 19, Macrophage Inflammatory Protein 3 betamip3b mip-3b mip3beta mip-3beta
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