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Invitrogen™ SLC (CCL21) Human Uncoated ELISA Kit with Plates Non-distribution product as customer accommodation.

Uncoated ELISA Kit with Plates

Manufacturer:  Invitrogen™ 885821422

Catalog No. 50-112-5173


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Includes: Capture Antibody Concentrate
Detection Antibody Concentrate
Human CCL21/SLC Standard (lyophilized): 5200pg/mL upon reconstitution
Coating Buffer Concentrate
Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)
Sample Diluent Z
pre-titrated Streptavidin-HRP
Tetramethylbenzidine (TMB) Substrate Solution

Description

Description

The Human Secondary Lymphoid-tissue Chemokine (CCL21) (SLC) Uncoated ELISA Kit contains pre-matched antibody pairs, plates, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human SLC. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).
Specifications

Specifications

O00585
Biotin
6366
Colorimetric Microplate Reader
ELISA Kit
RUO
Plasma,Serum,Supernatant
Plasma,50 μL|Serum,50 μL|Supernatant,50 μL
2-8°C
24 hr 30 min
CCL21
HRP
2,600-40.6 pg/mL
ELISA
96-well plates
10X Coating Buffer: Buffer for plating the Capture Antibody
2 x 96 tests
40.6 pg/mL
50μL
Human
1 hr 45 min
Human
Chemokine ligand 21, secondary lymphoid tissue chemokine
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