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Invitrogen™ MCP-3/CCL7 Human Uncoated ELISA Kit with Plates Non-distribution product as customer accommodation.

Uncoated ELISA Kit with Plates

Manufacturer:  Invitrogen™ 885070022

Catalog No. 50-112-5166


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Includes: Capture Antibody Concentrate
Detection Antibody Concentrate
Human CCL7( MCP-3) Standard (lyophilized): 100pg/mL upon reconstitution
Coating Buffer Concentrate
Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)
Sample Diluent Z
pre-titrated Streptavidin-HRP
Tetramethylbenzidine (TMB) Substrate Solution

Description

Description

The Human Monocyte Chemoattractant Protein 3 (CCL7) MCP-3) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human MCP-3. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
Specifications

Specifications

P80098
Biotin
6354
Colorimetric Microplate Reader
ELISA Kit
RUO
Plasma,Serum,Supernatant
Plasma,50 μL|Serum,50 μL|Supernatant,50 μL
2-8°C
24 hr 30 min
CCL7
HRP
0.3125-20 pg/mL
ELISA
96-well plates
10X Coating Buffer: Buffer for plating the Capture Antibody
2 x 96 tests
0.3125 pg/mL
50μL (perdiluted 1:2)
Human
1 hr 45 min
Human
FIC, MARC, Monocyte chemotactic protein 3
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