Invitrogen MCP-3/CCL7 Human Uncoated ELISA Kit with Plates
Uncoated ELISA Kit with Plates
Manufacturer: Invitrogen 885070022
The Human Monocyte Chemoattractant Protein 3 (CCL7) MCP-3) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human MCP-3. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
|Colorimetric Microplate Reader|
|Plasma,50 μL|Serum,50 μL|Supernatant,50 μL|
|24 hr 30 min|
|10X Coating Buffer: Buffer for plating the Capture Antibody|
|2 x 96 tests|
|50μL (perdiluted 1:2)|
|1 hr 45 min|
|FIC, MARC, Monocyte chemotactic protein 3|
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