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Invitrogen™ MCP-3/CCL7 Human ELISA Kit Non-distribution product as customer accommodation.

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ BMS2125

Catalog No. 50-112-5344


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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human MCP-3
Biotin-Conjugate anti-human MCP-3 monoclonal antibody
Human MCP-3 Standard lyophilized, 40pg/mL upon reconstitution
Streptavidin-HRP
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

Description

Description

The Human Monocyte-specific Chemokine 3 (CCL7)(Hu MCP-3) ELISA quantitates Hu MCP-3 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MCP-3. Principle of the method The Human MCP-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
Specifications

Specifications

P80098
Biotin
6354
Adhesive Plate Covers
96 tests
0.1 pg/mL
50μL
Human
1 hr 20 min
Human
FIC, MARC, Monocyte chemotactic protein 3
5.5%
0.3-20.0 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA Kit
RUO
Plasma,Serum,Supernatant
Plasma,50 μL|Serum,50 μL|Supernatant,50 μL
2-8°C
3 hr 15 min
CCL7
4.2%
HRP
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