Gibco™ Human CD366 (TIM3) Fc Recombinant Protein, PeproTech®

Description

Recombinant Protein

PeproTech's CHO cell-derived Recombinant Human TIM-3 Fc is a glycosylated, disulfide-linked homodimer of 412-amino-acid-residues whose monomer consists of a 179-amino acid portion of the extracellular domain fused to the 231-amino-acid length Fc portion of human IgG by two glycine residues. The calculated molecular weight of monomeric CHO cell-derived Recombinant Human TIM-3 Fc is 45.9 kDa; however, due to glycosylation, it migrates at an apparent molecular weight of approximately 180-200 kDa by SDS-PAGE analysis under non-reducing conditions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin, mucin-dmain containing-3) is a 281 amino acid long, Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. TIM3 consists of an IgV-like domain, a mucin-like domain in the extracellular region, and a conserved Tyrosine phosphorylation motif in the cytoplasmic region. TIM3 is involved in macrophage activation and induction of autoimmune diseases. Further, TIM3 down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance. Pathological significance of TIM3 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease, and also enhances the severity of experimental autoimmune encephalomyelitis in mice.

Specifications

• Accession Number: Q8TDQ0
• For Use With (Application): Functional Assay
• Format: Lyophilized
• Formulation: protein with no preservative
• Gene ID (Entrez): 84868
• Molecular Weight (g/mol): 45.9 kDa
• Name: Human CD366 (TIM3) Fc
• Purification Method: HPLC, SDS-PAGE
• Quantity: 10 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: CD366; FLJ14428; Havcr2; HAVcr-2; Hepatitis A virus cellular receptor 2; hepatitis A virus cellular receptor 2 homolog; kidney injury molecule-3; KIM-3; sCD366; soluble CD366; soluble TIM 3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain containing 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; Tim3; TIM-3; TIMD3; TIMD-3
• Common Name: TIM-3
• Gene Symbol: Havcr2
• Biological Activity: Determined by its ability to bind Recombinant human Galectin-9 in. a functional ELISA assay.
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: Monomer: SEVEYRAEVG QNAYLPCFYT PAAPGNLVPV CWGKGACPVF ECGNVVLRTD ERDVNYWTSR YWLNGDFRKG DVSLTIENVT LADSGIYCCR IQIPGIMNDE KFNLKLVIKP AKVTPAPTRQ RDFTAAFPRM LTTRGHGPAE TQTLGSLPDI NLTQISTLAN ELRDSRLAND LRDSGATIRG GPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK
• Content And Storage: -20°C
• Activity: Determined by its ability to bind Recombinant human Galectin-9 in. a functional ELISA assay.
• Expression System: CHO cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.