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Abnova™ Human CDK8 (NP_001251.1, 1 a.a. - 464 a.a.) and CCNC (NP_005181.2, 1 a.a. - 283 a.a.) Full-length Recombinant Protein with GST-tag expressed in baculovirus infected Sf21 cells

Used for Func, SDS-PAGE

Manufacturer:  Abnova™ P5517

Catalog No. 89-939-060


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Description

Description

The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Specifications

Specifications

Baculovirus infected insect cell (Sf21) expression system
Functional Study, Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis
Liquid
80kDa
Baculovirus infected insect cell (Sf21) expression system
91% by SDS-PAGE/CBB staining
5 ug
RUO
CDK8
The activity was determined by ELISA. The enzyme was incubated with biotinylated substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into streptavidin- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG. Substrate: RNA polymerase peptide. ATP: 100 uM.
Yes
NP_001251.1 (Gene ID : 1024);NP_005181.2 (Gene ID : 892)
Liquid
1024/892
CDK8/CCNC (Human) Recombinant Protein
Glutathione sepharose chromatography
Loading 1 ug protein in SDS-PAGE
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
K35/MGC126074/MGC126075
CDK8
Insect
GST
SDS
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Product Certifications
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