Gibco™ Human CXCL12 (SDF-1g) Recombinant Protein, PeproTech®

Recombinant Protein

Supplier:  Gibco™ 30028G100UG

Catalog No. 50-238-7937

Description

The SDF-1 proteins are stromal-derived CXC chemokines that signal primarily through the CXCR4 receptor. SDF-1 proteins exert chemotactic activity over a broad range of immune system cell types and may also be involved in other processes including metastasis of certain cancers, inflammation, and angiogenesis. Additionally, SDF-1 can suppress HIV entry into cells expressing the CXCR4 receptor. Five isomers of SDF-1 have been found with SDF-1alpha being the predominant form, expressing in a wide range of cell types. The SDF-1gamma isoform is distinctive in that it contains a highly basic C-terminal region, which presumably is the reason for its high binding affinity to gylcosaminoglycans (GAG) and heparin sulfate (HS). Consequently, most of SDF-1gamma exists in a cell- and membrane-bound form, which confers greater stability compared to other isoforms via increased resistance to proteolytic degradation. SDF-1gamma binds with reduced affinity to the CXCR4 receptor when compared to the alpha and beta isoforms, but because of its increased stability can, over time, exert equal or greater activity when compared to other SDF-1 isoforms. SDF-1gamma expression has been found primarily in the heart (human and mouse) and in the brain and central nervous system (rat).

Specifications

• Accession Number: P48061
• Format: Lyophilized
• Gene ID (Entrez): 6387
• Name: CXCL12
• Quantity: 100 μg
• Regulatory Status: RUO
• Gene Alias: PBSF, Stromal-Cell Derived Factor-1
• Product Type: Protein
• Conjugate: Unconjugated
• Sequence: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKGR REEKVGKKEK IGKKKRQKKR KAAQKRKN
• Expression System: E. coli

• For Use With (Application): Functional Assay
• Formulation: Protein with no preservative
• Molecular Weight (g/mol): 11.6 kDa
• Purification Method: HPLC, SDS-PAGE
• Storage Requirements: -20°C
• Endotoxin Concentration: <1 EU/μg
• Gene Symbol: CXCL12
• Specific Reactivity: Human
• Recombinant: Recombinant
• Activity: Determined by its ability to chemoattract human T cells.
• Purity or Quality Grade: ≥98% by SDS-PAGE gel and HPLC analyses.