Invitrogen™ HSP70 Human ELISA Kit

Sandwich ELISA Kit

Supplier:  Invitrogen™ BMS2087

Catalog No. 50-112-5289

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human HSP70
Biotin-Conjugate anti-human HSP70 monoclonal antibody

Description

The Human Heat Shock Protein 70 (Hu HSP70) ELISA quantitates Hu HSP70 in human serum, plasma, cell lysates or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HSP70. Principle of the method The Human HSP70 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity.

Specifications

• Accession Number: P34932
• Assay Sensitivity: 0.052 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: APG-2,HEL-S-5a,HS24/P52,HSPH2,RY,hsp70,hsp70RY
• Intraassay CV: 5.3%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr.

• Assay Range: 0.156-10.0 ng/mL
• Conjugate: Biotin
• Sample Type: Cell Lysates, Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 3308
• Interassay CV: 4.5%
• Kit Contents: Pre-coated 96 well plates, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Sample Volume: Cell Lysate,50 μL; Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Synonym: Heat Shock Protein 70
• Test Time: 1 hr. 20 min.