Invitrogen™ IFN beta Human ELISA Kit

Measure levels of human interferon beta (IFNb) in human cell and tissue culture media supernatant samples using 96-well plates.

Supplier:  Invitrogen™ 414101

Catalog No. PI414101

Description

Assay IFN-b (human interferon beta) levels in human cell and tissue culture media (supernatants) with theseInvitrgen™ Human IFN beta ELISA Kits.

Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by Natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit the development of tumors. IFN-beta binding results in the activation of the tyrosine kinases Jak1 and Tyk2, phosphorylation of members of the STAT family of transcription factors, and the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.

Specifications

• Accession Number: P01574
• Assay Sensitivity: 50 pg/mL
• Conjugate: HRP
• Product Type: ELISA Kit
• Specificity: No significant cross-reaction with human IFN-a or IFN-g; mouse or rat IFN-ß
• Target Species: Human
• Gene ID (Entrez): 3456
• Interassay CV: <8%
• Kit Contents: Pre-coated 96 well Microplate, Detection Antibody Concentrate, Anti-IgG-HRP Concentrate, Concentrate Diluent, Standard, Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Adhesive Plate Covers
• Measurement: Absorbance at 450nm
• Quantity: 96 Tests
• Sample Volume: Supernatant, 50 μL
• Target: Human
• Total Assay Time: 3 hr. 15 min.

• Assay Range: 50-4000 pg/mL
• Description: Human IFN beta ELISA Kit
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Sample Type: Supernatant
• Sufficient For: 96 microplate wells
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IFB,IFF,IFN-beta,IFNB
• Intraassay CV: <8%
• Label or Dye: HRP
• Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.