Assay IFN-b (human interferon beta) levels in human cell and tissue culture media (supernatants) with theseInvitrgen Human IFN beta ELISA Kits.These human IFN beta ELISA kits use pre-titered, matched pairs of coating and detection antibodies to accurately measure interferon beta (IFN-β) in samples at 100µL per well. These IFN-β kits are validated for use with human cell and tissue culture media. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
Target: Human interferon beta (human IFN-β, IFN-beta)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well plate
Measurement: Absorbance at 450nm
Assay Range: 25 to 2000pg/mL
Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Types: Cell culture supernatant, tissue culture media
Total Assay Time: 3.25 hours
Sample Size: 100µL
Specificity: No significant cross-reaction with human IFN-α or IFN-γ; mouse or rat IFN-β
Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, substrates, and stop solution.
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|Colorimetric ELISA (TMB substrate) in clear 96-well plate|
|Absorbance at 450nm|
|Human IFN beta ELISA Kit|
|Adhesive Plate Covers|
|Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%|
|No significant cross-reaction with human IFN-a or IFN-g; mouse or rat IFN-ß|
|3 hr. 15 min.|
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