Invitrogen™ IFN gamma Human ELISA Kit

Measure levels of human interferon gamma (IFNg) in human serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples using 96-well plates.

Supplier:  Invitrogen™ EHIFNG5

Catalog No. ENEHIFNG5

Description

Assay IFN-g (human interferon gamma) levels in human serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Invitrgen™ Human IFN gamma ELISA Kits.

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

Specifications

• Accession Number: P01579
• Assay Sensitivity: <2 pg/mL
• Conjugate: Biotin
• Product Type: ELISA Kit
• Specificity: No significant cross-reaction with with human IL-1a, IL-1ß, IL-2, IL-3, IL-6, IL-7, IL-8, TNFa or GM-CSF
• Target Species: Human
• Gene ID (Entrez): 3458
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, 30X Wash Buffer, 200mL
• Measurement: Absorbance at 450nm minus absorbance at 550nm
• Quantity: 5 x 96 Tests
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Target: Human
• Total Assay Time: 3 hr.

• Assay Range: 25.6-1000 pg/mL
• Description: Human IFN gamma ELISA Kit
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 5 x 96 microplate wells
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IFG,IFI
• Intraassay CV: <10%
• Label or Dye: HRP
• Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.