Invitrogen™ Mouse VEGF-165 Recombinant Protein, eBioscience™

Recombinant Protein

Supplier:  Invitrogen™ BMS359

Catalog No. 50-185-98

Description

VEGF (vascular endothelial growth factor) which is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. VEGF has a variety of effects on vascular endothelium, including the ability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability. The VEGF family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis, and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. Studies have shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis. Elevated levels of VEGF is linked to POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes) also known as Crow-Fukase syndrome which affects multiple organs in the body. Description: Vascular Endothelial Cell Growth Factor (VEGF) is a homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis and capillary permeability. Mouse VEGF is a 39 kDa homodimeric protein consisting of two 165 amino acid polypeptide chains. Purity: >95%. Molecular Weight: 39.0 kDa.

Specifications

• Accession Number: Q00731
• Formulation: protein with no preservative
• Molecular Weight (g/mol): 39.0kDa
• Quantity: 10μg
• Storage Requirements: -20°C
• Structural Form: E. Coli
• Gene Symbol: Vegfa
• Product Type: Recombinant Protein
• Conjugate: Unconjugated
• Species: E. coli
• Form: Lyophilized
• Purity or Quality Grade: Affinity chromatography

• For Use With (Application): Control
• Gene ID (Entrez): 22339
• Name: Mouse VEGF
• Source: E. coli
• Regulatory Status: RUO
• Common Name: Mouse VEGF
• Biological Activity: The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0 – 5.0ng/mL.
• Specific Reactivity: Mouse
• Cross Reactivity: Ms
• Recombinant: Recombinant
• Protein Subtype: Recombinant

Safety and Handling

ShelfLife : 12 Months