Human IgE Isotype Control (HE1), Invitrogen™

Human Isotype Control

Supplier:  Thermo Scientific DIAHE1A01

Catalog No. PIDIAHE1A01

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

DIA HE1-01A contains non-immune purified human IgE with kappa light chains produced in vitro from a monoclonal hybridoma. Original material is obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B, but must be handled as potentially infectious as all human material.The purity of this human IgE is >;98%, tested by SDS-PAGE. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The human IgE is purified by Protein L chromatography. The remaining contaminants are mainly components from Foetal Bovine Serum.This antibody is well suited as a standard in IgE quantifying assays due to its very low batch-to-batch variation. Other applications include immunochemistry and cellular immunology research.NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/ml

Specifications

• Antigen: Human IgE
• Clone: HE1
• Conjugate: Unconjugated
• Gene Alias: IgE
• Immunogen: Human IgE (non-immune)
• Quantity: 100 μL
• Primary or Secondary: Control
• Content And Storage: Store at -20°C, Avoid Freeze/Thaw Cycles
• Isotype: IgE κ

• Applications: Control, Immunohistochemistry
• Concentration: 1mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Host Species: Human
• Purification Method: Protein L
• Regulatory Status: RUO
• Target Species: Not Applicable
• Form: Liquid