Invitrogen™ Human IgG (Total) ELISA Kit

Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human total IgG

ELISA

The Human Total Immunoglobulin G (Hu IgG) ELISA quantitates Hu IgG in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu IgG. Principle of the method The Human IgG solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Human IgG, also known as human immunoglobulin G, is an antibody that is produced by the immune system in response to antigens. It is the most abundant immunoglobulin in the blood and plays a key role in defense against pathogens. IgG works by binding to specific antigens and promoting their clearance from the body through various mechanisms, including phagocytosis and complement activation. IgG is also involved in other immune responses, such as antibody-dependent cellular cytotoxicity and neutralization of toxins. Human IgG can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious and autoimmune diseases.

Specifications

• Assay Range: 1.6 to 100 ng/mL
• Assay Sensitivity: 0.24 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: 2.6%
• Intraassay CV: 5.3
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL
• Storage Requirements: 2°C to 8°C
• Synonym: Immunoglobulin G
• Target: Human IgG
• Test Time: 1 hrs 20 mins
• Total Assay Time: 1 hrs 30 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov