Human IgM Isotype Control, Invitrogen™

Human Isotype Control

Supplier:  Thermo Scientific 31146

Catalog No. PI31146

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.Purity: The precipitin lines against goat anti-human whole serum are the same as those against goat anti-human IgM, Fc5-mu fragment specific, when assayed by immunoelectrophoresis at an antigen concentration equivalent to 20 mg/ml. No reaction was observed against goat anti-human IgG, Fc fragment specific, goat anti-human IgA, alpha chain specific, or goat anti-human alpha-2-macroglobulin. This antibody is recommended for use in blocking assays, immunohistochemical, immunocytochemical and immunofluorescent applications.Storage: Store product at 4°C until opened. After opening, dilute only enough antibody for a single day's use. Store remainder at 4°C under sterile conditions.Country of Origin: USA

Specifications

• Antigen: Human IgM
• Concentration: 4.5mg/mL
• Formulation: PBS with no preservative; pH 7.6
• Host Species: Human
• Quantity: 2 mg
• Primary or Secondary: Control
• Content And Storage: 4° C
• Isotype: IgM

• Applications: Blocking Assay, Control, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
• Conjugate: Unconjugated
• Gene Alias: IgM, IgM mu
• Purification Method: Purified
• Regulatory Status: RUO
• Target Species: Not Applicable
• Form: Liquid