Invitrogen IL-18 Human Instant ELISA Kit
Instant ELISA Kit
Manufacturer: Invitrogen BMS267INST
The Human Interleukin 18 (Hu IL-18) ELISA quantitates Hu IL-18 in human serum, plasma, cell culture supernatants, urine or other body fluids. The assay will exclusively recognize both natural and recombinant Hu IL-18. Principle of the method The Human IL-18 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.
|Adhesive Plate Covers|
|Interleukin-18, Interferon-gamma-inducing factor|
|Colorimetric Microplate Reader|
|Plasma, Serum, Supernatants, Urine|
|Plasma (50μL), Serum (50μL), Supernatants (50μL), Urine (50μL)|
|3 hr 10 min|
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