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Invitrogen™ IL-28A Human Uncoated ELISA Kit with Plates Non-distribution product as customer accommodation.

Uncoated ELISA Kit with Plates

Manufacturer:  Invitrogen™ 885210222

Catalog No. 50-112-5167


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Includes: Capture Antibody Concentrate (250 x)
Detection Antibody Concentrate (250 x)
human IL-28 standard, (lyophilized), 2000pg/mL upon reconstitution
Phosphate Buffered Saline Concentrate (PBS) 10x
Assay Buffer A Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Sample Diluent Z
Tetramethylbenzidine (TMB) Substrate Solution
2 96-well plates

Description

Description

The Human Interleukin 28A (IL-28A) Uncoated ELISA Kit contains pre-matched antibody pairs ,plates, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-28A. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-28A belongs to the IFN lambda family, a novel family of cytokines within the IL-10 superfamily. The three members of this family are IL-29 (IFN lambda 1), IL-28A (IFN lambda 2), and IL-28B (IFN lambda 3), and are also known as the type III Interferons.The IFN lambdas signal through a heterodimeric receptor of which one subunit, IL-10R2, is shared with other members of the superfamily. The second subunit, IFN lambda R1 or IL-28R alpha, is unique to the IFN lambdas. Signaling occurs through the Jak/STAT pathway in a similar manner as the type I IFN (IFN alpha/beta) and activates many of the same genes despite low sequence homology between the cytokines and receptors in the two families. Both IFN families display antiviral activity through the induction of antiviral protein production in target cells and the upregulation of MHC class I expression. These proteins also exhibit antiproliferative and antitumor effects, making them a possible alternative to IFN alpha cancer therapies. Unlike the type I IFN, which are able to stimulate most cells, response to IFN lambda stimulation appears to be limited to dendritic and some tumor cells due to the limited expression of IFN lambda R1. Another notable difference is the ability of the IFN lambda stimulation to drive dendritic cells towards the production of CD4+CD25+FoxP3+ regulatory T cells, suggesting a possible immunoregulatory role.
Specifications

Specifications

Q8IZJ0
Biotin
282616
Colorimetric Microplate Reader
ELISA Kit
RUO
Plasma,Serum,Supernatant
Plasma,50 μL|Serum,50 μL|Supernatant,50 μL
2-8°C
24 hr 30 min
IFNL2
HRP
15.6-1,000 pg/mL
ELISA
96-well plates
10X Coating Buffer: Buffer for plating the Capture Antibody
2 x 96 tests
15.6 pg/mL
50μL
Human
1 hr 45 min
Human
IFNL2, IFNL3 Interleukin 28, IL-28A, IL-28B
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