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Invitrogen™ IL-8 Human Instant ELISA™ Kit Non-distribution product as customer accommodation.

Instant ELISA Kit

Manufacturer:  Invitrogen™ BMS2043INST

Catalog No. 50-179-41


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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-8/NAP-1, Biotin-Conjugate (anti-human IL-8/NAP-1 polyclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
Aluminium pouch(es) with a human IL-8/NAP-1 Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Sample Diluent (Use when an external predilution of the samples is needed)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

Description

Description

The Human Interleukin 8 (Hu IL-8) ELISA quantitates Hu IL-8 in human serum, plasma, cell culture supernatants, amniotic fluid or other body fluids. The assay will exclusively recognize both natural and recombinant Hu IL-8. Principle of the method The Human IL-8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-8 (Interleukin-8) is a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.
Specifications

Specifications

HRP
ELISA Kit
15.6-1,000 pg/mL
3576
RUO
-20°C
3 hr. 10 min.
Protein 3-10C, CXCL8, NAP-1, GCP-1
8.1%
Human
1.3 pg/mL
Colorimetric Microplate Reader
Biotin
P10145
ELISA
Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard curve strips, Wash Buffer Concentrate, Substrate Solution, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
Amniotic Fluid, 50 μL|Plasma, 50 μL|Serum, 50 μL|Supernatant, 50 μL
20 min.
Human
10.8%
128 Tests
Amniotic Fluid, Plasma, Serum, Supernatant
GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1
SDS
Documents
Product Certifications
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