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Abnova™ Human IL4 (130 a.a.) Full-length Recombinant Protein with His tag expressed in Barley grain

Used for Func, SDS-PAGE, WB-Re

Manufacturer:  Abnova™ P5823

Catalog No. 89-948-912

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.



The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]



Human IL4 (130 a.a.) Full-length Recombinant Protein with His tag expressed in Barley grain
>95% by SDS-PAGE
BCGF-1, BCGF1, BSF1, IL-4, MGC79402
His Tag
Barley grain (Hordeum vulgare) expression system
Lyophilized from PBS, pH7,2.
SDS-PAGE Stained with Coomassie Blue
Store at −20°C on dry atmosphere.After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at −20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Aliquot to avoid repeated freezing and thawing.
<0.005ng/μg protein (0.05EU/μg) as measured by turbidimetric kinetic assay
The bioactivity is determined by the cell proliferation assay for IL4 for dose-dependent cell proliferation of human TF-1 cells. The ED50 value for this effect is typically < 0.3 ng/mL, corresponding to a specific activity > 3.3 x 10e6 units/mg. Optimal concentration should be determined for specific applications and cell lines.
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