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Abnova™ Human LATS1 (NP_004681.1, 589 a.a. - 1130 a.a.) Partial Recombinant Protein with GST-tag expressed in baculovirus infected Sf21 cells

Used for Func, SDS-PAGE

Manufacturer:  Abnova™ P5573

Catalog No. 89-950-426


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Description

Description

The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. [provided by RefSeq]

Specifications

Specifications

NP_004681.1
Functional Study, Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis
Liquid
90kDa
Baculovirus infected insect cell (Sf21) expression system
66% by SDS-PAGE/CBB staining
5 ug
RUO
LATS1
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Srctide. ATP: 1000 uM
Yes
Baculovirus infected insect cell (Sf21) expression system
Liquid
9113
LATS1 (Human) Recombinant Protein
Glutathione sepharose chromatography
Loading 1 ug protein in SDS-PAGE
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
WARTS|wts
LATS1
Insect
GST
SDS
Documents
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