Abnova™ Human LATS1 (NP_004681.1, 589 a.a. - 1130 a.a.) Partial Recombinant Protein with GST-tag expressed in baculovirus infected Sf21 cells

Used for Func, SDS-PAGE

Supplier:  Abnova™ P5573

Catalog No. 89-950-426

Description

The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. [provided by RefSeq]

Specifications

• Accession Number: NP_004681.1
• Formulation: Liquid
• Molecular Weight (g/mol): 90kDa
• Preparation Method: Baculovirus infected insect cell (Sf21) expression system
• Quality Control Testing: Loading 1 ug protein in SDS-PAGE
• Storage Requirements: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Gene Alias: WARTS/wts
• Gene Symbol: LATS1
• Species: Baculovirus
• Protein Tag: GST
• Form: Liquid

• For Use With (Application): Functional Study, SDS-PAGE
• Gene ID (Entrez): 9113
• Name: LATS1 (Human) Recombinant Protein
• Purification Method: Glutathione sepharose chromatography
• Quantity: 5 ug
• Regulatory Status: RUO
• Common Name: LATS1
• Biological Activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Srctide. ATP: 1000 uM
• Recombinant: Recombinant
• Expression System: Baculovirus infected insect cell (Sf21) expression system
• Purity or Quality Grade: 66% by SDS-PAGE/CBB staining