R&D Systems™ Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Supplier:  R&D Systems™ 8989T2050

Catalog No. 89-89T-2050

Description

Measured by its binding ability in a functional ELISA. When Recombinant Human LILRB1/CD85j/ILT2 is coated at 2 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN ) binds with a typical ED50 of 0.075-0.45 μg/mL.

Specifications

• Accession Number: Q8NHL6
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Molecular Weight (g/mol): M.W. (Observed): 65-82 kDa, reducing conditions; M.W. (theoretical): 48 kDa
• Source: Human embryonic kidney cell, HEK293-derived human LILRB1/CD85j/ILT2 protein Human LILRB1 (Gly24-His458) (N-terminus) Accession # Q8NHL6 HHHHHH (C-terminus)
• Structural Form: Monomer
• Gene Alias: CD85 antigen-like family member J, CD85J, CD85j antigen, Ig-like transcript 2, ILT2, ILT-2, ILT2FLJ37515, Immunoglobulin-like transcript 2, leukocyte Ig-like receptor-1, Leukocyte immunoglobulin-like receptor 1, leukocyte immunoglobulin-like receptor subfamily B member 1, leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform, leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), LIR1, LIR1LIR-1MIR-7CD85MIR7CD85 antigen, member 1, MIR7, Monocyte/macrophage immunoglobulin-like receptor 7
• Gene Symbol: LILRB1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Expression System: Human embryonic kidney cell, HEK293-derived Human LILRB1 (Gly24-His458) Accession # Q8NHL6, N-terminus; HHHHHH, C-terminus
• Protein: ILT2/CD85j/LILRB1

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 10859
• Quantity: 50 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Common Name: ILT2/CD85j/LILRB1
• Biological Activity: Measured by its binding ability in a functional ELISA. When Recombinant Human LILRB1/CD85j/ILT2 is coated at 2μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 binds with a typical ED50 of 75-450ng/mL.
• Species: Human
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.