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Invitrogen™ MIF Human ELISA Kit
Sandwich ELISA Kit
Supplier: Invitrogen™ EHMIF
Description
The Human Macrophage Inflammatory Factor (Hu MIF) ELISA quantitates Hu MIF in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MIF. Principle of the method The Human MIF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
MIF (Macrophage Migration Inhibitory Factor) is a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through suppression of the anti-inflammatory effects of glucocorticoids. MIF may also play a role in integrin signaling pathways, because it has been found to form a complex in the cytosol near the peripheral plasma membrane with the JAB1 protein.Specifications
P14174 | |
6 pg/mL | |
Solid-phase sandwich ELISA | |
ELISA Kit | |
Plasma, Serum, Supernatant | |
96 assays | |
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
4282 | |
<12% | |
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
96 Tests | |
Immunology | |
2°C to 8°C | |
Human | |
4 hr. 45 min. |
8.23-6000 pg/mL | |
6pg/mL to 6000pg/mL | |
Biotin | |
Cytokines & Receptors | |
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. | |
Human | |
Colorimetric Microplate Reader | |
MIF | |
<10% | |
HRP | |
RUO | |
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL | |
GIF, GLIF, MMIF | |
1 hr. 20 min. |
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