Invitrogen™ NRG1 beta-1 Human ELISA Kit

Sandwich ELISA Kit

Supplier:  Invitrogen™ EHNRG1

Catalog No. EHNRG1

Description

The Human NRG1beta-1 (Hu NRG1) ELISA quantitates Hu NRG1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NRG1. Principle of the method The Human NRG1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Neuregulin-1 beta 1/NRG1 beta 1 is one of many soluble and transmembrane NRG1 isoforms. All family members share an EGF-like domain that interacts with the ErbB family of tyrosine kinase receptors. NRG1 isoforms can be classified into type I (neu differentiation factor, NDF; heregulin, HRG; acetylcholine receptor inducing activity, ARIA), type II (glial growth factor, GGF) and type III (sensory and motor neuron-derived factor, SMDF) isoforms. NRG1 regulates multiple nervous system functions including neurotransmitter expression, axon guidance, synapse formation and plasticity, glial cell development, and axon myelination. It regulates heart morphogenesis and contractility and also plays a cardioprotective role following tissue injury. Multiple polymorphisms and aberrant expression of NRG1 isoforms are associated with the development of schizophrenia and many cancers.

Specifications

• Accession Number: Q02297
• Assay Sensitivity: 50 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 3084
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Extracellular Matrix & Cell Adhesion
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 82.3-20,000 pg/mL
• Calibration Range: 50pg/mL to 20000pg/mL
• Conjugate: Biotin
• Protein Family: activation of transmembrane receptor protein tyrosine kinase activity; cardiac conduction system development; cardiac muscle cell differentiation; cardiac muscle cell myoblast differentiation; cell communication; cell migration; cell morphogenesis; cell proliferation; cellular protein complex disassembly; embryo development; endocardial cell differentiation; epidermal growth factor receptor signaling pathway; ERBB signaling pathway; Fc-epsilon receptor signaling pathway; fibroblast growth factor receptor signaling pathway; glial cell fate commitment; innate immune response; locomotory behavior; mammary gland development; MAPK cascade; negative regulation of cardiac muscle cell apoptotic process; negative regulation of extrinsic apoptotic signaling pathway in absence of ligand; negative regulation of protein catabolic process; negative regulation of secretion; negative regulation of transcription, DNA-templated; nervous system development; neural crest cell development; neuron fate comm
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: ARIA,GGF,GGF2,HGL,HRG,HRG1,HRGA,MST131,MSTP131,NDF-IT2,SMDF,NRG1
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
• Synonym: ARIA, GGF, GGF2, HGL, HRG, HRG1, HRGA, MST131, MSTP131, NDF-IT2, SMDF, NRG1
• Test Time: 1 hr. 20 min.