Invitrogen™ Osteoactivin (GPNMB) Human ELISA Kit

Sandwich ELISA Kit

Supplier:  Invitrogen™ EHGPNMB

Catalog No. EHGPNMB

Description

The Human Osteoactivin (GPNMB) ELISA quantitates Hu GPNMB in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu GPNMB. Principle of the method The Human GPNMB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.

Specifications

• Accession Number: Q14956
• Assay Sensitivity: 45 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 10457
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Extracellular Matrix & Cell Adhesion
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 49.15-12,000 pg/mL
• Calibration Range: 45pg/mL to 12,000pg/mL
• Conjugate: Biotin
• Protein Family: Cell Structure & Organelle Proteins
• Specificity: This pair antibody detects human Osteoactivin. It show less than 20% cross-reactivity with recombinant mouse Osteoactivin.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: HGFIN,NMB
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 10 μL; Serum, 10 μL; Supernatant, 100 μL
• Synonym: UNQ1725/PRO9925, HGFIN, NMB
• Test Time: 1 hr. 20 min.