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R&D Systems™ Human PlGF, Carrier-Free Recombinant Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Supplier: R&D Systems™ 264PGB010/CF
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DescriptionMeasured by its binding ability in a functional ELISA. When Recombinant Human VEGF R1/Flt-1 Fc Chimera (Catalog # 3516-FL ) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Recombinant Human PlGF that produces 50% of the optimal binding response is approximately 0.75-4.5 ng/mL.
|Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA. Reconstitute at 200μg/mL in 4 mM HCl.|
|Observed M.W.: 12-15kDa, reducing conditions, Theoretical M.W.: 15kDa|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.|
|D12S1900, PGF, PGFL, placenta growth factor, placental growth factor, placental growth factor, vascular endothelial growth factor-related protein, PlGF, PlGF-2, PLGFplacental growth factor-like, SHGC-10760|
|>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining|
|E. coli-derived human PlGF protein Ala21-Arg149|
|<0.10 EU per 1μg of the protein by the LAL method.|
|When Recombinant Human VEGF R1/Flt-1 Fc Chimera is immobilized at 0.5μg/mL, 100μL/well, the concentration of Recombinant Human PlGF that produces 50% of the optimal binding response is approximately 0.15-0.9ng/mL.|
|E. coli-derived Ala21-Arg149|
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