Invitrogen™ RANTES Human ELISA Kit

Measure levels of regulated upon activation, normal T-cell expressed and secreted (RANTES) in human serum, plasma, tissue extracts, urine and culture supernatant samples.

Supplier:  Invitrogen™ EHRNTS

Catalog No. ENEHRNTS

Description

Assay RANTES (regulated upon activation, normal T-cell expressed and secreted) levels in human serum, plasma, tissue extracts, urine and culture supernatant samples with our Invitrgen™ Human RANTES ELISA Kits.

These human RANTES ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of RANTES (regulated upon activation, normal T-cell expressed and secreted) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: Natural and recombinant human RANTES (regulated upon activation, normal T-cell expressed and secreted)

• Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

• Measurement: Absorbance at 450nm minus absorbance at 550nm

• Assay Range: 51.2 to 2000pg/mL

• Sensitivity: < 2pg/mL

• Precision: Intra-assay CV < 10%; inter-assay CV < 10%

• Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

• Total Assay Time: 3 hours

• Sample Size: 50μL

• Specificity: No significant cross-reaction with human Eotaxin, GROα, GROβ, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3, MCP-4, G-CSF, GM-CSF, IFNα, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-16, IL-17, TNFα or TNFβ, or rat RANTES

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

• Accession Number: P13501
• Assay Sensitivity: 2 pg/mL
• Conjugate: Biotin
• Product Type: ELISA Kit
• Specificity: No significant cross-reaction with human Eotaxin, GROa, GROß, MIP-1a, MIP-1ß, MCP-1, MCP-2, MCP-3, MCP-4, G-CSF, GM-CSF, IFNa, IFNg, IL-1a, IL-1ß, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-16, IL-17, TNF
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: D17S136E,RANTES,SCYA5,SIS-delta,SISd,TCP228,eoCP
• Intraassay CV: <10%
• Label or Dye: HRP
• Precision: Intra-assay CV <10%; inter-assay CV <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

• Assay Range: 51.2 to 2000pg/mL
• Description: Human RANTES ELISA Kit
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Sample Type: Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum, Urine
• Sufficient For: 96 microplate wells
• Gene ID (Entrez): 6352
• Interassay CV: <12%
• Kit Contents: TMB Substrate Solution, 13mL, 30X Wash Buffer, 50mL, Streptavidin-HRP Concentrate, 75µL, Adhesive Plate Covers, 6 ea, Anti-Human RANTES Precoated 96-well Strip Plate, 1 ea, HRP Dilution Buffer, 14mL, Lyophilized Recombinant Human RANTES Standard, 2 vials, Standard Diluent, 2 x 25mL, Stop Solution, 13mL, Biotinylated Antibody Reagent, 8mL
• Measurement: Absorbance at 450nm minus absorbance at 550nm
• Quantity: 96 Tests
• Sample Volume: Plasma,1μL; Serum,1μL; Supernatant,50μL; Urine,50μL
• Target: Human
• Total Assay Time: 3 hr.