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Invitrogen™ eBioscience™ Human sE-selectin Platinum ELISA Kit Non-distribution product as customer accommodation.

For the quantitative detection of human sE-selectin.

Manufacturer:  Invitrogen™ BMS205

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Catalog No. 50-179-60


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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sE-selectin
HRP-Conjugate anti-human sE-selectin monoclonal antibody
Human sE-selectin Standard lyophilized, 100ng/mL upon reconstitution
Control high, lyophilized
Control low, lyophilized
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

Description

Description

E-selectin (CD62E, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. E-selectin is highly expressed on activated endothelial cells (IL-2, TNF- alpha, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. E-selectin is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. E-selectin binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162). The HCD62E antibody has been shown to recognize human E-selectin and to be useful for flow cytometry. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
Specifications

Specifications

P16581
HRP
6401
Pre-coated 96 well plate, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Controls
96 tests
0.3 ng/mL
20μL
Human
1 hr 20 min
Human
ELAM1, endothelial adhesion molecule 1, CD62E
5.4%
1.6-50 ng/mL
ELISA
Colorimetric Microplate Reader
ELISA
RUO
Plasma, Serum, Supernatants
Plasma (20μL), Serum (20μL), Supernatants (20μL)
2-8°C
2 hr 10 min
SELE
6%
HRP
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