Invitrogen™ Serpin A1 Human ELISA Kit

Sandwich ELISA Kit

Supplier:  Invitrogen™ EHSERPINA1

Catalog No. EHSERPINA1

Description

The Human Serpin A1 ELISA quantitates Hu Serpin A1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Serpin A1. Principle of the method The Human Serpin A1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of alpha-1-AT, these enzymes are not inhibited and they may digest pulmonary parenchyma. Alpha-1-AT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in alpha-1-AT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.

Specifications

• Accession Number: P01009
• Assay Sensitivity: 2 ng/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 5265
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Immunology
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 2.05-500 ng/mL
• Calibration Range: 2ng/mL to 500ng/mL
• Conjugate: Biotin
• Protein Family: Proteases, Peptidases & Inhibitors
• Specificity: This ELISA pair antibody detects human Serpin A1. Other species not determined.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: A1A,A1AT,AAT,PI,PI1,PRO2275,alpha1AT
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 33 μL; Serum, 33 μL; Supernatant, 100 μL
• Synonym: PRO0684, A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT
• Test Time: 1 hr. 20 min.