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Human TGF beta 3 Carrier-Free, Recombinant Protein, eBioscience™ Non-distribution product as customer accommodation.

Recombinant Protein

Manufacturer:  Invitrogen 34873482

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Catalog No. 50-112-3524



Description

Description

Transforming growth factor beta 3 (TGF beta 3) is the third member of the transforming growth factor family of cytokines, which also includes TGF beta 1 and beta 2. These cytokines are secreted in precursor form consisting of a bioactive C-terminal domain attached to an N-terminal domain known as latency associated protein (LAP). Cleavage of LAP results in the mature protein, which functions as a disulfide-linked homodimer. As with all members of the family, TGF beta 3 is highly conserved across species, with mouse and human TGF beta 3 demonstrating 100% sequence homology and cross-species activity.The members of this family can be expressed by most cell types and exert pleiotropic effects, which include the suppression of B- and T-cell effector activity, mediation of tissue healing, and suppression of tumor proliferation. The promotion of CD4+CD25+ T-cell expansion is a newly discovered function of the TGF beta cytokines, and indicates an important role in the protection against autoimmunity. Description: Transforming growth factor beta 3 (TGF beta 3) is the third member of the transforming growth factor family of cytokines, which also includes TGF beta 1 and beta 2. These cytokines are secreted in precursor form consisting of a bioactive C-terminal domain attached to an N-terminal domain known as latency associated protein (LAP). Cleavage of LAP results in the mature protein, which functions as a disulfide-linked homodimer. As with all members of the family, TGF beta 3 is highly conserved across species, with mouse and human TGF beta 3 demonstrating 100% sequence homology and cross-species activity. The members of this family can be expressed by most cell types and exert pleiotropic effects, which include the suppression of B- and T-cell effector activity, mediation of tissue healing, and suppression of tumor proliferation. The promotion of CD4+CD25+ T-cell expansion is a newly discovered function of the TGF beta cytokines, and indicates an important role in the protection against autoimmunity. Applications Reported: Recombinant human TGF beta 3 is biologically active and can inhibit IL-4-induced proliferation of mouse HT-2 cells. Applications Tested: The ED50 of this protein, as measured by inhibition of IL-4-induced proliferation of mouse HT-2 cells, is less than or equal to 0.05ng/mL, which corresponds to a specific activity of greater than 2 x 10^7 Units/mg. Source: E. coli (amino acids Ala301-Ser412; accession # NP_003230.1). Bioactivity: The ED50 of this protein, as measured by inhibition of IL-4-induced proliferation of mouse HT-2 cells, is less than or equal to 0.05ng/mL, which corresponds to a specific activity of greater than 2 x 10e7 Units/mg. Endotoxin: Less than 0.1ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE and HPLC. Molecular Weight: 25.0 kDa. Storage and handling: For best recovery, quick spin vial prior to opening. Reconstitute in 5-10 mM citric acid to a concentration of 0.1-1.0mg/mL. Do not vortex. Use in sterile environment. Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier-protein (e.g., 0.1% BSA) at less than or equal to -20°C.
Specifications

Specifications

P10600
Lyophilized
7043
Human TGF beta 3 Carrier-Free
Affinity chromatography
E. coli
RUO
Recombinant
Human TGF beta 3 Carrier-Free
Control, Functional Assay, Neutralization
Recombinant Protein, Carrier-Free
Unlabeled
E. coli
Control, Functional Assay, Neutralization
protein with 0.1% TFA and no preservative
25.0kDa
SDS-PAGE and HPLC
100μg
-20°C
E. coli (amino acids Ala 301 - Ser412; Accession # NP_003230.1)
Less than 0.1ng/μg cytokine as determined by the LAL assay
TGFB3
The ED50 of this protein, as measured by inhibition of IL-4-induced proliferation of mouse HT-2 cells, is less than or equal to 0.05ng/mL, which corresponds to a specific activity of greater than 2 x 107 Units/mg.
Human
Hu
Recombinant
Safety and Handling

Safety and Handling

ShelfLife : 12 Months

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