HuR Mouse, Human, PE, Clone: 3A2, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 566341

Catalog No. BDB566341

Description

The 3A2 monoclonal antibody specifically recognizes HuR (Human antigen R) which is encoded by ELAVL1 /(ELAV like RNA binding protein 1). HuR belongs to the ELAVL (embryonic lethal, abnormal vision and Drosophila-like) family of proteins that includes HuB ( ELAVL2 /), HuC ( ELAVL3 /) and HuD ( ELAVL4 /). These proteins contain three RNA recognition motifs that selectively bind AU rich elements (AREs) found in the 3' untranslated regions of messenger RNAs. Although predominantly located in the nucleus, HuR shuttles between the nucleus and cytoplasm. HuR stabilizes ARE-containing mRNAs, regulates RNA splicing, and can thus influence the outcome of gene expression. HuR is expressed by a variety of cell types including leucocytes, eg, T cells, B cells, and dendritic cells, whereas the other ELAVL family members are primarily expressed by neurons. HuR is involved in regulating cellular growth and differentiation, eg, through the stabilization and shuttling of mRNAs including those encoding transcription factors, proto-oncogenes, costimulatory receptors, growth factors, cytokines, chemokines, and inflammatory mediators. Abnormal expression of HuR has been linked to a number of diseases, for example, overexpressed HuR levels have been detected in certain tumor cells. The 3A2 antibody crossreacts with mouse HuR as well as with human HuB and HuD, but not with HuC.

R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.

Specifications

• Antigen: HuR
• Clone: 3A2
• Conjugate: PE
• Gene Alias: HUR, ELAVL1, ELAV1, Hua, MelG
• Immunogen: Human HuR Recombinant Protein
• Quantity: 25 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse
• Isotype: IgG1 κ