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Hygromycin B, 50mg/mL in distilled water, sterile-filtered, Alfa Aesar™

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Quantity:
5 x 1mL
31282-04-9
C20H37N3O13
527.524
MFCD06795479
GRRNUXAQVGOGFE-BBMONYMYSA-N
134129613
(3'S,3aS,4S,4'S,5'R,6R,6'R,7R,7aS)-4-[(1R,2S,3R,5S,6S)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
CNC1CC(C(C(C1O)OC2C3C(C(C(O2)CO)O)OC4(O3)C(C(C(C(O4)C(CO)N)O)O)O)O)N
31282-04-9
C20H37N3O13
527.524
MFCD06795479
GRRNUXAQVGOGFE-BBMONYMYSA-N
134129613
(3'S,3aS,4S,4'S,5'R,6R,6'R,7R,7aS)-4-[(1R,2S,3R,5S,6S)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
CNC1CC(C(C(C1O)OC2C3C(C(C(O2)CO)O)OC4(O3)C(C(C(C(O4)C(CO)N)O)O)O)O)N
Applications
Hygromycin B was used for hygro resistance effect in the following cells transfected 293T cells with mixture of plasmids at 160 μg/ml, MCF-7ALOX12 cells at 150 μg/ml, MSCV Luciferase PGK-hygro plasmid and STAT3 Reporter HeLa Stable Cell Line (stably transfected with Luciferase gene under the control of STAT3 promoter) maintained in DMEM supplemented with 10% FBS. It is suitable for the selection of cells transfected with Escherichia coli hygromycin resistance gene (hyg or hph), as well as for the maintenance of the Hygromycin phenotype of resistant cells. Consistent with its more specific effect on translocation, hygromycin B exhibits monophasic inhibition of polypeptide synthesis, suggesting that it binds to a single site on the ribosome.

Solubility
Fully miscible in water and also soluble in methanol or ethanol and buffer solution.

Notes
Protect from heat. Keep the container tightly closed and place it in a cool, dry and well ventilated condition. Store in freezer at -20°C.
UN Number UN2810
Physical Form Liquid
Concentration 50mg/mL in distilled water
Quantity 5 x 1mL
Chemical Name or Material Hygromycin B, sterile-filtered

GHS H Statement
H301-H330-H334-H318-H312-H317
Toxic if swallowed.
Fatal if inhaled.
May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Causes serious eye damage.
Harmful in contact with skin.
May cause an allergic skin reaction.

GHS P Statement
P260-P301+P310-P304+P340-P305+P351+P338-P320-P330-P405-P501a
Do not breathe dust/fume/gas/mist/vapors/spray.
IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Specific treatment is urgent (see label).
Rinse mouth.
Store locked up.
Dispose of contents/container in accordance with local/regional/national/international regulations.

Danger

DOTInformation : Transport Hazard Class: 6.1; Packing Group: III; Proper Shipping Name: TOXIC, LIQUIDS, ORGANIC, N.O.S.

RTECSNumber : WK2130000

storageNote1 : −30° to −10°C