Thermo Scientific™ Hypersil GOLD™ PREP C8 HPLC Columns

Get exceptional peak shape and resolution in HPLC and LC/MS using generic gradients. Use for analytes with medium hydrophobicity or when less hydrophobicity than C18 is needed.

Catalog Number	Mfr. No.	Particle Size	Diameter (Metric)	Length (Metric)
03-253-817	Thermo Scientific™ 25205018823A	5 μm	10 mm	8 mm
03-253-818	Thermo Scientific™ 25205019023A	5 μm	10 mm	20 mm
03-253-815	Thermo Scientific™ 25205259070A	5 μm	10 mm	250 mm
03-253-816	Thermo Scientific™ 25205259270A	5 μm	20 mm	250 mm
03-253-954	Thermo Scientific™ 25205259270XL	5 μm	25 mm	250 mm

Description

Maintain the sensitivity you need for your purification needs using Thermo Scientific™ Hypersil GOLD™ C8 preparative LC Columns. Best for scale up from analytical to prep LC method and ideal for analytes with medium hydrophobicity or when a less hydrophobic phase is required to obtain optimum retention. The lower hydrophobicity allows compounds to elute more quickly, yielding faster separations. Benefit from similar selectivity to C18 columns with a predictable elution order. Our columns come in a variety of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed.

Similar selectivity, less retention than C18 columns

• Successful retention and separation of moderately hydrophobic analytes.
• Best for fatty acids, which are too hydrophobic for analysis using C18 columns.
• C8 (USP L7) selectivity.

Faster Separations

• Higher analytical throughput compared to C18 alkyl chain chemistry.
• Faster compound elution due to reduced hydrophobic interactions.

Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs.

Standard Threaded and Standard Flange Hardware

• Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use.
• Standard threaded hardware for column i.d. < 40mm.
• Standard flange hardware for column i.d. ≥ 40mm.
• Standard hardware can be modified on request to be compatible with SFC.

XtendedLife Hardware

• Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.
• Compatible with SFC and is available in dimensions up to 50mm i.d.

Preparative Guards are a proven and cost effective way to extend the lifetime of your prep column. Our preparative guard columns require the use of a preparative guard holder.

Customer Specific Orders

• If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in a preparative scale not shown or would like to discuss a specific column dimension, we would be happy to discuss your requirements.
• Please contact your local sales representative for Customer Specific Orders.
• Custom built columns are not eligible for returns.

About the Hypersil GOLD family of HPLC columns

Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. Twelve different chemistries are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.

Specifications

• Particle Size: 5 μm
• Pore Size: 175 Å
• Temperature: 60°C
• Stationary Phase: C8
• Carbon Load: 8%
• Endcapped: Yes

• pH: 2 to 9
• Surface Area: 220 m²/g
• USP Type: L7
• Packing Material: Spherical, Fully Porous, Ultrapure Silica
• Column Type: Reversed Phase
• Phase: Reversed Phase