I-Ak Mouse anti-Mouse, Brilliant Violet 711, Clone: 10-3.6, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 743475

Catalog No. BDB743475

Description

The 10-3.6 monoclonal antibody specifically recognizes theβchain of the I-A[k] MHC class II alloantigen. It cross-reacts with splenocytes from mice of the H-2[f], H-2[r], and H-2[s] haplotypes, from nonobese diabetic (NOD, H-2[g7]) mice, and with lymph node cells of BN, DA, WRA, and WRC rats. Reactivity with other haplotypes ( e.g., b, d, p, q /) has not been observed. Long-term in vivo /treatment with 10-3.6 mAb has been reported to prevent the spontaneous development of autoimmune insulin-dependent diabetes mellitus (IDDM) in female NOD mice.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm. This dye offers a very bright choice for the violet laser. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.

Specifications

• Antigen: I-Ak
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: Aβk
• Immunogen: C3H mouse splenocytes
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV711 under optimal conditions that minimize unconjugated dye and antibody.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Murine

• Clone: 10-3.6
• Conjugate: Brilliant Violet 711
• Format: Affinity Purified
• Host Species: Murine
• Isotype: Mouse CWB IgG_2a, κ
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal