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ID2 Mouse anti-Human, Mouse, PE-Cyanine7, Clone: ILCID2, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  LIFE TECHNOLOGIES 25947582

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Catalog No. 25-947-582


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Description

Description

Description: This ILCID2 monoclonal antibody recognizes mouse and human ID2. This ILCID2 antibody requires Foxp3/Transcription Factor Staining Buffer set and will perform poorly when used with standard IC Fixation & Permeabilization Buffer Set and protocol. Applications Reported: This ILCID2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This ILCID2 antibody has been tested by flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product No. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No.

00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser The inhibitor of DNA binding 2 (ID2) protein is essential for promoting the development of a number of cell types including natural killer cell, erythroid precursors, as well as, CD8+ and CD4+ T-cell. A lack of ID2 promotes B-cell development. The chief role of this protein is to associate with ubiquitously-expressed E proteins, and other proteins of the basic helix-loop-helix (HLH) family, preventing them from binding to DNA and activating transcription. Activation of ID2 is critical for the development of the natural killer cell lineage.
Specifications

Specifications

ID2
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P41136, Q02363
Ac2-300; AI255428; BHLHB26; C78922; GIG8; Id-2; ID2; ID2A; ID2H; Idb2; inhibitor of DNA binding 2
Mouse
IgG1, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
15902, 3398
Flow Cytometry
ILCID2
PE-Cyanine7
ID2
Liquid
ID2
Purified recombit fragment of human ID2, corresponding to amino acids 1-134 expressed in E. coli
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse
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