IDO Monoclonal Antibody (eyedio), Brilliant Ultra Violet™ 737, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
36-794-7742	LIFE TECHNOLOGIES 367947742	100 Tests
50-255-0846	LIFE TECHNOLOGIES 367947741	25 Tests

Description

This eyedio monoclonal antibody reacts with human indoleamine-2,3-dioxygenase (IDO, INDO, IDO1), an intracellular enzyme that catalyzes the degradation of tryptophan to kynurenines. IDO is expressed in a wide variety of tissues and cells, including macrophages, plasmacytoid dendritic cells, and several cell lines. IDO can be induced in many different cell types by IFN gamma or other inflammatory stimuli. Expression of IDO in antigen presenting cells and tumors is thought to mediate immune suppression through depletion of this essential amino acid and/or by the creation of tryptophan metabolites that cause apoptosis of T cells and induction of regulatory T cells. Applications Reported: This eyedio antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This eyedio antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Brilliant Ultraviolet 737 is a tandem dye that emits at 732 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser.

IDO Monoclonal antibody specifically detects IDO in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: IDO
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P14902
• Gene ID (Entrez): 3620
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Gene: Ido1

• Clone: eyedio
• Applications: Flow Cytometry
• Conjugate: Brilliant Ultraviolet 737
• Host Species: Mouse
• Target Species: Human
• Gene Alias: EC 1.13.11.52; IDO; IDO1; IDO-1; INDO; indolamine 2,3 dioxygenase; indole 2,3-dioxygenase; indoleamine; indoleamine 2,3-dioxygenase 1; indoleamine 23-dioxygenase; Indoleamine-2; indoleamine-pyrrole 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase
• Gene Symbols: Ido1
• Primary or Secondary: Primary
• Product Type: Antibody