Invitrogen™ IFN beta Human ELISA Kit

Measure levels of human interferon beta (IFNb) in human cell and tissue culture media supernatant samples using 96-well plates.

Catalog Number	Mfr. No.	Quantity
PI414101	Invitrogen™ 414101	96 Tests

Description

Assay IFN-b (human interferon beta) levels in human cell and tissue culture media (supernatants) with theseInvitrgen™ Human IFN beta ELISA Kits.

These human IFN beta ELISA kits use pre-titered, matched pairs of coating and detection antibodies to accurately measure interferon beta (IFN-β) in samples at 100μL per well. These IFN-β kits are validated for use with human cell and tissue culture media. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: Human interferon beta (human IFN-β, IFN-beta)

• Format: Colorimetric ELISA (TMB substrate) in clear 96-well plate

• Measurement: Absorbance at 450nm

• Assay Range: 25 to 2000pg/mL

• Sensitivity: Undetermined

• Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

• Sample Types: Cell culture supernatant, tissue culture media

• Total Assay Time: 3.25 hours

• Sample Size: 100μL

• Specificity: No significant cross-reaction with human IFN-α or IFN-γ; mouse or rat IFN-β

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, substrates, and stop solution.

Specifications

• Description: Human IFN beta ELISA Kit
• Specificity: No significant cross-reaction with human IFN-a or IFN-g; mouse or rat IFN-ß
• Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Measurement: Absorbance at 450nm